Live-dead assays are essential tools for quickly sorting out drugs and concentrations based on toxicity, and gain understanding of drug mechanisms.
Because they are ironically toxic to cells, live-dead stains only capture a snapshot of cell viability and miss critical kinetic information. Saguaro's non-toxic live-dead stain makes it possible to track cell viability and cytotoxicity in real-time, and gain better understanding of toxic liabilities and drug mechanism.
LIVE-Dead Stain™ Features
Non-Toxic
Non-perturbing to cells, allowing real-time quantification of cell health over multiple days
Mix-and-Read
One-step, no-wash dye which remains in media throughout the assay.
Fast and sensitive
Use both green and red fluorescence to quickly and sensitively discriminate live from dead cells
Live-Dead Stain FAQ
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Saguaro's Live-Dead Stain uses the following two fluorescent channels (exc/em): 500nm / 520nm (dead), and 665nm / 680nm (live).
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Cells that have their cellular membrane compromised are stained in green, and are therefore considered dead, whereas living cells will be stained in red and loose their red fluorescence when dead.